Tissue Procurement Shared Core Service
The Tissue Procurement Core provides University of Colorado Cancer Center members and other funded researchers with well-characterized human tumors and materials derived from human tumors for research in human cancer. Our organ-based pathology subspecialists contribute anatomic expertise to organizing collection protocols and verify histopathological classification of specimens we collect.
We provide investigators with IRB-approved protocols and pathologiy subspecialist collaborators with:
- frozen tissue fragments
- tissue sections
- DNA
- RNA
- cells purified by microdissection from tumors, preneoplastic lesions and matched normal control tissue
Services We Provide
- Tissue grossing
- Tissue processing
- Tissue embedding
- Cutting paraffin and frozen sections
- HE staining
- Cell line fixation, including cell pallet creation
- Tissue array block creation from donor blocks
- Tissue array cutting
- Special cutting and staining sections for microdissection
- Immunohistochemistry staining, including antibody standardizations
- Tunnel assay staining
- Some histology special stains, upon request
Turnaround time depends on the specimen or project. Please contact Jenny Caputo for a time estimate for your project.
Equipment
- Tissue-Tek Vacuum Infiltration Processor
- Microm Tissue Embedding Unit
- Microm Paraffin Microtome
- Shandon Varistain Slide Stainer
- Dako Autostainer Automated Immunostaining System
- Biocare Medical Decloaking Chamber for Antigen Retrieval
- Fisher Tissue Prep Flotation Bath for Paraffin Sections
- Thermo Shandon Slide Heater-Oven
- Fisher Isotemp 205 Water Bath for heating up reagents
- Fisher Isotemp Table Top Oven for Reagent Incuvation
- Forma Liquid Nitrogen Freezer with Inventory Storage System