Laser Capture Shared Core Service

What is Laser Capture Microdissection (LCM)?

Laser capture microdissection (LCM) is technique for obtaining pure cells from specific tissue sections. A machine uses a laser beam to activate a special transfer film. The film bonds to cells that have been identified and targeted by microscopy.

Laser capture microdissection (LCM) is a technique that allows pathologists to lift specifically targeted cells from a section of cell tissue, leaving behind unwanted cells that could contaminate the molecular purity of subsequent analysis. This technology, which was developed in the late 1990s at NIH in collaboration with bioengineering and cancer pathology groups, is precise, accurate and automated.

LCM uses a special laser and film, which absorbs the laser energy and leaves the macromolecules undamaged. Starting tissue can be fixed, embedded in paraffin or frozen.

Why Use LCM in Gene Expression Studies?

To reveal accurate, cell-type specific expression profiles that otherwise might be obscured in mixed cell samples.

Technology isolates homogeneous populations of cells from tissue sections    

• Cells can be collected based on morphologic or immunohistologic features
• Machine can recognize color (stains) or fluorescence 
• Cells are harvested for DNA, RNA or Protein analysis

AutoPix LCM Machine

The AutoPix allows you to select all cells or areas to be captured automatically, instead of manual firing.

• You can train the machine to recognize cells based on morphology or immunohistochemical or fluorescent staining. We can also store customized recognition programs.
• The machine's optical upgrade provides improved visualization of non-cover-slipped tissue sections, allowing for better cell-type recognition.
• You can focus the laser to capture single cells, or use a broader area to capture cell clusters.
• We provide technical assistance and reagents for dealing with LCM-captured material for subsequent molecular analysis.