University of Colorado Cancer Center Gene Expression Shared Core Service

Based on the expression of 415 genes differentially expressed in gefitinib-resistant/gefitinib-sensitive NSCLC, hierarchical clustering using uncentered correlation and complete linkage shows that HNSCC and NSCLC cell lines with similar gefitinib response sort together. Red, gefitinib-resistant HNSCC; green, gefitinib-sensitive HNSCC; blue, gefitinib-resistant NSCLC; pink, gefitinib-sensitive NSCLC

HNSCC and NSCLC cell lines with similar
gefitinib response sort together.

The Gene Expression Core is dedicated to providing state-of-the-art facilities and technical support and services for analysis of gene expression, which will allow UCCC members to be on the cutting edge of cancer research.

The Gene Expression Core is comprised of:

Core Objectives

  • Analyze gene expression using both pre-made and custom designed oligonucleotide arrays
  • Facilitate gene discovery
  • Accurately quantitate gene expression using real time PCR and GenomeLab GeXP technology
  • Provide support for data analysis and bioinformatics by our data mining tool and our data analysis capabilities
  • Remain committed to using a variety of approaches for expression analysis
  • Provide consultation and educational services to help those members with limited knowledge on microarray technology and to bring the Core customers up to date as technology advances

The Gene Expression Core was established in response to the technological advances in gene array, as well as the demand for high throughput expression analysis and genomic investigation to investigate pathogenesis, therapeutics, genetic susceptibility and gene discovery in cancer research. The informatics aspect is of paramount importance, and five distinct analysis programs, as well as a network, are used to store, share and analyze the data.

To date, the Core is one of the highest volume Affymetrix microarray facilities in academia in the country, having performed more than 10,900 microarrays including test arrays over seven years for nearly 250 investigators. On average, 85% of the users are UCCC members.

NCICC

Gene Expression Core

Locations

Micro Array

9th Avenue Campus
Belle Bonfils Bldg, Room 3B10
4200 E. 9th Ave.
Denver, Colo. 80262
Fax: 303-315-2698

Anschutz Medical Campus
RC1-South, Room 6213
12801 E. 17th Pl.
Aurora, CO 80045
Fax: 303-724-3365

PCR Service

Anschutz Medical Campus
RC1-South, Room 7212
303-724-3965

Leadership & Staff

Mark Geraci, MD
Core Director
mark.geraci@ucdenver.edu
303-315-0398

Bryan Haugen, MD
Core Co-Director
PCR Director
bryan.haugen@ucdenver.edu
303-724-3921

Bifeng Gao, PhD
Microarray Core Manager
bifeng.gao@ucdenver.edu
303-724-3367

Uma Pugazhenthi
PCR Core Manager
umarani.pugazhenthi@ucdenver.edu
303-724-3965

Okyong Cho, MA
PRA, Anschutz Microarray
okyong.cho@ucdenver.edu
303-724-3367

Ted Shade, MCIS
PRA, 9th Avenue Microarray
ted.shade@ucdenver.edu
303-315-4492

Todd Woessner, BS
PRA, 9th Ave. Microarray
Brian.Woessner@ucdenver.edu
303-315-2624