Flow Cytometry Protocols

Krishan Stain for DNA Cell Cycle Analysis

  1. Prepare a single cell suspension with 0.1 to 1 x 10-6 cells per tube
  2. Wash cells with PBS, then centrifuge to pellet them.
  3. Remove the supernatant PBS
  4. Vortex to dislodge the pellet
  5. Add 1 ml of Krishan’s stain (link to recipe)
  6. Vortex again
  7. Refrigerate and let stand at least overnight before analysis

Saponin/PI Stain for Apoptosis & DNA Cell Cycle Analysis

  1. Prepare a single cell suspension with 0.1 to 1.0 x 10-6 cells per tube
  2. Wash cells with PBS, then centrifuge to pellet them.
  3. Remove the supernatant PBS
  4. Vortex to dislodge the pellet
  5. Add 1 ml of sponin/PI stain (link to recipe)
  6. Vortex again
  7. Refrigerate and let stand at 6 to 24 hours before analysis, but no longer than 24 hours

Luminex Multiplex Assays

Flow Cytometry

Location

RC-1 South, Room 4215
Anschutz Medical Campus
303-724-3145

Mailing Address

UCCC Flow Cytometry Lab
PO Box 6511, Mail Stop 8127
Aurora, CO 80045

Leadership & Staff

Christopher J. Hogan , PhD, Director
chris.hogan@ucdenver.edu

Karen Helm, Manager
karen.helm@ucdenver.edu

Christine Childs, PRA
christine.childs@ucdenver.edu

Thomas Sullivan, PRA
thomas.sullivan@ucdenver.edu