Flow Cytometry Protocols

Krishan Stain for DNA Cell Cycle Analysis

1. Prepare a single cell suspension with 0.1 to 1 x 10-6 cells per tube
2. Wash cells with PBS, then centrifuge to pellet them.
3. Remove the supernatant PBS
4. Vortex to dislodge the pellet
5. Add 1 ml of Krishan’s stain (link to recipe)
6. Vortex again
7. Refrigerate and let stand at least overnight before analysis

Saponin/PI Stain for Apoptosis & DNA Cell Cycle Analysis

1. Prepare a single cell suspension with 0.1 to 1.0 x 10-6 cells per tube
2. Wash cells with PBS, then centrifuge to pellet them.
3. Remove the supernatant PBS
4. Vortex to dislodge the pellet
5. Add 1 ml of sponin/PI stain (link to recipe)
6. Vortex again
7. Refrigerate and let stand at 6 to 24 hours before analysis, but no longer than 24 hours

Luminex Multiplex Assays

• Biosource 
• Biorad
• Luminex