Flow Cytometry Services at the University of Colorado Cancer Center

The Flow Cytometry Core at the University of Colorado Cancer Center offers services to Cancer Center members, Charles C. Gates Regenerative Medicine and Stem Cell Biology Program members, academic non-members and private industry researchers. CC and RMSCB Members receive scheduling priority and a discounted rate for services. Learn more about becoming a UCCC member or a RMSCB member. (links to member pages)

Our experienced staff can analyze your samples for you or train you to run the analysis equipment for all services except cell sorting. Core staff performs all cell sorting.

Flow Cytometry Analysis Services

We have three main analyzers, each best for different types of analysis. You may use the machines yourself or ask us to analyze your samples for you.

Becton Dickinson FACSCalibur

• 2 lasers: 488 and 635
• 4 color analysis
• Suggested fluors: FITC , PE , PECy7, APC

Beckman Coulter FC500

• High through-put
• 2 lasers: 488 and 635
• 5 color analysis
• Suggested fluors: FITC , PE , PE-Texas Red, APC, PECy7, PI

Moflo Cyan

• Analysis rate of 70,000 events/second
• 100 million event data files
• New 40mW red diode laser for enhanced APC and APC-Cy7 detection
• 9-color analysis
• 9 colors

Cell Sorting Services

We have two cell sorting machines with different capabilities. Core staff performs all cell sorts.

Moflo

• 3 lasers: 488, 635 and tunable Kripton
• Many laser lines for optimizing fluorescent protein detection
• Plate sorting
• 7 color capability

Moflo XDP

• Optimized for rare events
• Plate sorting
• 4 lasers: UV, 405, 488, 635
• 9 color capability
• Sort rate of 70,000 events per second
• 5 decade data resolution
• Plate sorting for any size plate
• 4-way sorting

Luminex Multiplex Bead Assays

This analysis measures cytokines, chemokines, phosphoproteins and apoptosis markers.

Also available

• Victor Multilabel Counter (self-service only in RC-1 South, Room 4215)
• Cytospin microscope slide centrifuge